CBIS法を用いた抗体作製のまとめ
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BINDSの加藤プロジェクトでは、高度化研究において、CBIS (Cell-Based Immunization and Screening)法の開発を実施中です。
これまでのCBIS法による抗体作製例を以下に列挙します（2021年11月14日現在; 30抗体）。
- 30. anti-HER3 (H3Mab-17)
Asano T, Ohishi T, Takei J, Nakamura T, Nanamiya R, Hosono H, Tanaka T, Sano M, Harada H, Kawada M, Kaneko MK, Kato Y.
Anti-HER3 monoclonal antibody exerts antitumor activity in a mouse model of colorectal adenocarcinoma
Oncol. Rep., 46(2): 173, https://doi.org/10.3892/or.2021.8124, 2021 (PDF)
- 29. anti-elephant PDPN (PMab-265)
Hosono H, Asano T, Takei J, Sano M, Tanaka T, Kaneko MK, Kato Y
Development of an Anti-Elephant Podoplanin Monoclonal Antibody PMab-265 for Flow cytometry
Monoclon. Antib. Immunodiagn. Immunother., 40(3), 141–145, DOI: 10.1089/mab.2021.0015, 2021 (PDF)
- 28. anti-California Sea Lion PDPN (PMab-269)
Tanaka T, Asano T, Sano M, Takei J, Hosono H, Nanamiya R, Nakamura T, Yanaka M, Harada H, Fukui M, Suzuki H, Uchida K, Nakagawa T, Kato Y, Kaneko MK.
Development of Monoclonal Antibody PMab-269 Against California Sea Lion Podoplanin
Monoclon. Antib. Immunodiagn. Immunother., 40(3), 124–133, DOI: 10.1089/mab.2021.0011, 2021 (PDF)
- 27. anti-mCCR8 (C8Mab-2)
Tanaka T, Nanamiya R, Takei J, Nakamura T, Yanaka M, Hosono H, Sano M, Asano T, Kaneko MK, Kato Y.
Development of Anti-Mouse CC chemokine receptor 8 Monoclonal Antibodies for Flow Cytometry
Monoclon. Antib. Immunodiagn. Immunother.,40(2), 65–70, DOI: 10.1089/mab.2021.0005, 2021 (PDF)
- 26. anti-hKLRG1 (KLMab-1)
Asano T, Nanamiya R, Tanaka T, Kaneko MK, Kato Y.
Development of anti-human killer cell lectin-like receptor subfamily G member 1 monoclonal antibodies for flow cytometry
Monoclon. Antib. Immunodiagn. Immunother.,40(2), 76–80, DOI: 10.1089/mab.2021.0008, 2021 (PDF)
- 25. anti-hCCR9 (C9Mab-1)
Nanamiya R, Takei J, Asano T, Tanaka T, Sano M, Nakamura T, Yanaka M, Hosono H, Kaneko MK, Kato Y.
Development of Anti-Human CC Chemokine Receptor 9 Monoclonal Antibodies for Flow Cytometry
Monoclon. Antib. Immunodiagn. Immunother.,40(3), 101–106, DOI: 10.1089/mab.2021.0007, 2021 (PDF)
- 24. anti-hTIGIT (TgMab-2)
Takei J, Asano T, Nanamiya R, Nakamura T, Yanaka M, Hosono H, Tanaka T, Sano M, Kaneko MK, Harada H, Kato Y.
Development of Anti-Human T-Cell Immunoreceptor with Ig and ITIM Domains (TIGIT) Monoclonal Antibodies for Flow Cytometry
Monoclon. Antib. Immunodiagn. Immunother.,40(2),71–75, DOI: 10.1089/mab.2021.0006, 2021 (PDF)
- 23. anti-mCCR3 (C3Mab-2)
Asano A, Nanamiya R, Takei J, Nakamura T, Yanaka M, Hosono H, Tanaka T, Sano M, Kaneko MK, Kato Y.
Development of Anti-Mouse CC Chemokine Receptor 3 Monoclonal Antibodies for Flow Cytometry
Monoclon. Antib. Immunodiagn. Immunother., 40(3), 107–112, DOI: 10.1089/mab.2021.0009, 2021 (PDF)
- 22. anti-TROP2 (TrMab-29)
Sayama Y, Kaneko MK, Takei J, Hosono H, Sano M, Asano T, Kato Y.*
(*corresponding author)
Establishment of a novel anti-TROP2 monoclonal antibody TrMab-29 for immunohistochemical analysis
Biochem Biophys Rep.,25, 100902, https://doi.org/10.1016/j.bbrep.2020.100902, 2021 (PDF)
- 21. anti-TROP2 (TrMab-6)
Sayama Y, Kaneko MK, Kato Y.*
(*corresponding author)
Development and characterization of TrMab-6, a novel anti-TROP2 monoclonal antibody for antigen detection in breast cancer
Mol Med Rep. , 23(2), 92, https://doi.org/10.3892/mmr.2020.11731, 2021 (PDF)
- 20. anti-EpCAM (EpMab-16)
Hosono H, Ohishi T, Takei J, Asano T, Sayama Y, Kawada M, Kaneko MK, Kato Y.*
(*corresponding author)
The anti-EpCAM monoclonal antibody EpMab-16 exerts antitumor activity in a mouse model of colorectal adenocarcinoma
Oncol Lett., 20: 383, https://www.spandidos-publications.com/10.3892/ol.2020.12246, 2020 (PDF)
- 19. anti-sheep PDPN (PMab-253 and PMab-260)
Kaneko MK, Sano M, Takei J, Asano T, Sayama Y, Hosono H, Kobayashi A, Konnai S, Kato Y.*
(*corresponding author)
Development and Characterization of Anti-Sheep Podoplanin Monoclonal Antibodies PMab-253 and PMab-260
Monoclon. Antib. Immunodiagn. Immunother., 39(4):144–155 DOI: 10.1089/mab.2020.0018, 2020 (PDF)
- 18. anti-CD20 (C20Mab-60)
Furusawa Y, Kaneko MK, Kato Y*
(*corresponding author)
Establishment of an Anti-CD20 Monoclonal Antibody (C20Mab-60) for Immunohistochemical Analyses
Monoclon. Antib. Immunodiagn. Immunother.,39(4):112–116, DOI: 10.1089/mab.2020.0015, 2020 (PDF)
- 17. anti-sheep PDPN (PMab-256)
Kato Y*, Furusawa Y, Sano M, Takei J, Nakamura T, Yanaka M, Okamoto S, Handa S, Komatsu Y, Asano T, Sayama Y, Kaneko MK.
(*corresponding author)
Development of an Anti-Sheep Podoplanin Monoclonal Antibody PMab-256 for Immunohistochemical Analysis of Lymphatic Endothelial Cells
Monoclon. Antib. Immunodiagn. Immunother.>, 39(3):82–90; DOI: 10.1089/mab.2020.0005, 2020 (PDF)
- 16. anti-CD19 (C19Mab-1, C19Mab-3)
Yamada S, Kaneko MK, Sayama Y, Asano T, Sano M, Yanaka M, Nakamura T, Okamoto S, Handa S, Komatsu Y, Nakamura Y, Furusawa Y, Takei J, Kato Y.*
(*corresponding author)
Development of novel mouse monoclonal antibodies against human CD19
Monoclon. Antib. Immunodiagn. Immunother., 39(2);45-50; DOI: 10.1089/mab.2020.0003, 2020 (PDF)
- 15. anti-CD20 (C20Mab-11)
Furusawa Y, Kaneko MK, Kato Y.*
(*corresponding author)
Establishment of C20Mab-11, a novel anti-CD20 monoclonal antibody, for the detection of B cells
Oncol Lett., 20(2), 1961-1967; https://doi.org/10.3892/ol.2020.11753, 2020 (PDF)
- 14. anti-bear PDPN (PMab-241)
Takei J, Yamada S, Konnai S, Ishinazaka T, Shimozuru M, Kaneko MK, Kato Y.*
(*corresponding author)
PMab-241 Specifically Detects Bear Podoplanin of Lymphatic endothelial cells in the Lung of Brown Bear
Monoclon. Antib. Immunodiagn. Immunother., 38(6):282–284, https://doi.org/10.1089/mab.2019.0038, 2019 (PDF)
- 13. anti-goat PDPN (PMab-235)
Furusawa Y, Yamada S, Nakamura T, Sano M, Sayama Y, Itai S, Takei J, Harada H, Fukui M, Kaneko MK, Kato Y.
PMab-235: a monoclonal antibody for immunohistochemical analysis against goat podoplanin
Heliyon, 5(7), e02063, 2019 (PDF)
- 12. anti-whale PDPN (PMab-237)
Kato Y*, Furusawa Y, Itai S, Takei J, Nakamura T, Sano M, Harada H, Yamada S, Kaneko MK.
(*corresponding author)
Establishment of an anti-cetacean podoplanin monoclonal antibody PMab-237 for immunohistochemical analysis
Monoclon. Antib. Immunodiagn. Immunother., 38(3), 108–113, https://doi.org/10.1089/mab.2019.0013, 2019 (PDF)
- 11. anti-bear PDPN (PMab-247)
Furusawa Y, Takei J, Sayama Y, Yamada S, Kaneko MK, Kato Y.*
(*corresponding author)
Development of an anti-bear podoplanin monoclonal antibody PMab-247 for immunohistochemical analysis
Biochem Biophys Rep., Volume 18, July 2019, 100644, https://doi.org/10.1016/j.bbrep.2019.100644, 2019 (PDF)
- 10. anti-alpaca PDPN (PMab-225)
Kato Y*, Furusawa Y, Yamada S, Itai S, Takei J, Sano M, Kaneko MK.
(*corresponding author)
Establishment of a monoclonal antibody PMab-225 against alpaca podoplanin for immunohistochemical analyses
Biochem Biophys Rep., Volume 18, July 2019, 100633, https://authors.elsevier.com/sd/article/S2405580818303194, 2019 (PDF)
- 9. anti-Tasmanian devil PDPN (PMab-233)
Furusawa Y, Yamada S, Itai S, Nakamura T, Takei J, Sano M, Harada H, Fukui M, Kaneko MK, Kato Y. *
(*corresponding author)
Establishment of a monoclonal antibody PMab-233 for immunohistochemical analysis against Tasmanian devil podoplanin
Biochem Biophys Rep., Volume 18, July 2019, 100631, https://doi.org/10.1016/j.bbrep.2019.100631 (PDF)
- 8. anti-tiger PDPN (PMab-231)
Furusawa Y, Kaneko MK, Nakamura T, Itai S, Fukui M, Harada H, Yamada S, Kato Y.*
(*corresponding author)
Establishment of a monoclonal antibody PMab-231 for tiger podoplanin
Monoclon. Antib. Immunodiagn. Immunother., 38(2):89–95, https://doi.org/10.1089/mab.2019.0003, 2019 (PDF)
- 7. anti-horse PDPN (PMab-219)
Furusawa Y, Yamada S, Itai S, Nakamura T, Yanaka M, Sano M, Harada H, Fukui M, Kaneko MK, Kato Y.*
(*corresponding author)
PMab-219: a monoclonal antibody for the immunohistochemical analysis of horse podoplanin
Biochem Biophys Rep., 18:100616. doi: 10.1016/j.bbrep.2019.01.009., 2019 (PDF)
- 6. anti-pig PDPN (PMab-210)
Furusawa Y, Yamada S, Itai S, Sano M, Nakamura T, Yanaka M, Fukui M, Harada H, Mizuno T, Sakai Y, Takasu M, Kaneko MK, Kato Y*.
(*corresponding author)
PMab-210: A Monoclonal Antibody Against Pig Podoplanin
Monoclon. Antib. Immunodiagn. Immunother., 38(1), 30-36; https://doi.org/10.1089/mab.2018.0038, 2019 (PDF)
- 5. anti-pig PDPN (PMab-213)
Kato Y*, Yamada S, Furusawa Y, Itai S, Nakamura T, Yanaka M, Sano M, Harada H, Fukui M, Kaneko MK.
(*corresponding author)
PMab-213: a monoclonal antibody for immunohistochemical analysis against pig podoplanin
Monoclon. Antib. Immunodiagn. Immunother., 38(1), 18-24; https://doi.org/10.1089/mab.2018.0048, 2019 (PDF)
- 4. anti-CD44 (C44Mab-5)
Yamada Y, Itai S, Nakamura T, Yanaka M, Kaneko MK, Kato Y*.
(*corresponding author)
Detection of high CD44 expression in oral cancers using the novel monoclonal antibody, C44Mab-5
Biochem Biophys Rep.,14, 64-68, 2018 (PDF)
- 3. anti-PD-L1 (L1Mab-13)
Yamada S, Itai S, Nakamura T, Yanaka M, Chang YW, Suzuki H, Kaneko MK, Kato Y.*.
(*corresponding author)
Monoclonal Antibody L1Mab-13 Detected PD-L1 in Lung Cancers.
Monoclon. Antib. Immunodiagn. Immunother., doi: 10.1089/mab.2018.0004, , 37(2), 110-115, 2018 (PDF)
- 2. anti-cat PDPN (PMab-52)
Yamada S, Itai S, Nakamura T, Yanaka M, Saidoh N, Chang YW, Handa S, Harada H, Kagawa Y, Ichii O, Konnai S, Kaneko MK, Kato Y*.
(*corresponding author)
PMab-52: Specific and Sensitive Monoclonal Antibody against Cat Podoplanin for Immunohistochemistry.
Monoclon. Antib. Immunodiagn. Immunother., 36(5): 224-230, 2017 (PDF)
- 1. anti-CD133 (CMab-43)
Itai S, Fujii Y, Nakamura T, Chang YW, Yanaka M, Saidoh N, Handa S, Suzuki H, Harada H, Yamada S, Kaneko MK, Kato Y.
(*corresponding author)
Establishment of CMab-43, a Sensitive and Specific Anti-CD133 Monoclonal Antibody, for Immunohistochemistry.
Monoclon. Antib. Immunodiagn. Immunother., 36(5): 231-235, 2017 (PDF)
BINDS
