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[(\))-195.5(\()-5.4(Si)-5.6(gma)-6.6(-).3(A)-6.3(l).2(d)-5.5(r).3(ic)-6.6(h)-195.5(C)-.7(or)-5.4(p.,)-197.9(S).4(t)-5.6(.)-192.1(Lo)-5.5(ui)-5.6(s,)-192.1(M)-6.4(O)-.6(,)-197.9(U)-.6(SA)-6.4(\).)-197.9(Af)-5.4(ter)-195.5(a)]TJ
-2.3788 -1.1174 TD
.015 Tc
[(4-hour)-320.1(incuba)-4.5(tion,)-322.5(the)-327.1(C)1.4(alcei)]TJ
12.32 0 TD
.0128 Tc
[(n)-322.4(A)-6.4(M)-323.3(r)-5.5(ele)-6.7(a)-1(s)-5.4(e)-323.5(of)-328.1(su)-5.6(pe)-6.7(rn)-5.6(ata)-6.7(n).2(t)]TJ
-12.32 -1.1116 TD
.0149 Tc
[(from)-304.2(eac)-4.6(h)-303(w)1.4(ell)-303.1(w)1.4(a)-4.6(s)-302.8(mea)-4.6(sured.)-299.5(Fluoresc)-4.6(ence)-304.1(inte)-4.6(nsity)-303(w)-4.3(a)1.1(s)]TJ
T*
.0146 Tc
[(determined)-464.5(using)-458.8(a)-465.7(mic)-4.9(r)2(oplate)-465.7(re)-4.9(ader)-464.5(\(Pow)-4.6(er)-464.5(Sca)-4.9(n)-458.8(H)-4.6(T).8(;)]TJ
0 -1.1174 TD
.013 Tc
[(B)-6.4(i).3(o)-5.4(Tek)-304.9(In)-5.4(st)-5.5(rum)-6.6(ent)-5.5(s).6(,)-301.5(W)-1.9(i)-5.5(n).4(o)-5.4(o).4(s)-5.2(k).4(i)-5.5(,)-295.7(V)-6.2(T)-.8(,)-301.5(U)-.5(S)-5.2(A)-.5(\))-304.8(w)-.5(it)-5.5(h)-299.1(a)-.8(n)-304.9(e)-.8(xc)-6.5(it)-5.5(a-)]TJ
0 -1.1116 TD
.0127 Tc
[(t)-5.8(i)0(o)-5.7(n)-282.1(wav)-5.7(e)-1.1(l)-5.8(e)-1.1(ng)-5.7(th)-282.1(o)-5.7(f)-276.3(4)-5.7(8).1(5)-172.7(n).1(m)-283.4(a)-1.1(nd)-282.1(a)-6.8(n)-276.4(e)-6.8(m)-1.1(i)-5.8(s).3(s)-5.5(i)0(on)-282.1(w)-6.5(a)-1.1(ve)-6.8(le)-6.8(ngt)-5.8(h)-282.1(o).1(f)]TJ
T*
.0133 Tc
[(5)-5.1(3).7(8)-172.1(n).7(m.)-376(C)-6.1(y).7(t)-5.2(o).7(ly)-5.1(ti)-5.2(c)-374.9(a)-.5(c)-6.2(t).6(i)-5.2(v).7(it)-5.2(y)-373.7(\()-5(%)-374.8(o).7(f)-379.4(l).6(y)-5.1(s).9(i)-5.2(s).9(\))-373.7(w)-5.9(as)-379.3(cal)-5.2(cu)-5.1(la)-6.2(ted)-379.5(a)-.5(s)]TJ
0 -1.1174 TD
.3593 Tc
[(%l)340.8(y)346.7(s)341.1(i)346.6(s)]TJ
/F10 1 Tf
3.2658 0 TD
0 Tc
(=)Tj
/F3 1 Tf
.7372 0 TD
(\()Tj
/F8 1 Tf
.3456 0 TD
(E)Tj
/F10 1 Tf
.8006 0 TD
(-)Tj
/F8 1 Tf
.7315 0 TD
(S)Tj
/F3 1 Tf
.5126 0 TD
.0183 Tc
[(\)/)5.6(\()]TJ
/F8 1 Tf
.9964 0 TD
0 Tc
(M)Tj
/F10 1 Tf
1.0137 0 TD
(-)Tj
/F8 1 Tf
.7315 0 TD
(S)Tj
/F3 1 Tf
.5184 0 TD
(\))Tj
/F10 1 Tf
.5126 0 TD
()Tj
/F3 1 Tf
.7372 0 TD
.0126 Tc
[(10)-5.8(0,)-342.2(w)-6.6(h)0(e)-6.9(r)0(e)]TJ
/F8 1 Tf
5.0167 0 TD
0 Tc
(E)Tj
/F3 1 Tf
.9676 0 TD
.0129 Tc
[(i)-5.6(s)-345.1(u)-5.5(ore)-6.6(sc)-6.6(en)-5.5(ce)-346.5(of)]TJ
-16.8875 -1.1116 TD
.0143 Tc
[(c)-5.2(o)1.7(m)-5.3(b)1.7(ined)-332.4(target)-332.5(a)-5.2(n)1.7(d)-332.4(e)-5.2(ffe)-5.2(ctor)-332.3(c)-5.2(e).5(lls,)]TJ
/F8 1 Tf
15.0444 0 TD
0 Tc
(S)Tj
/F3 1 Tf
.8467 0 TD
.0126 Tc
[(is)-333.9(s)-5.6(p)0(o)-5.8(n)0(t)-5.9(a)-1.2(ne)-6.9(ou)-5.8(s)-333.9().1(u)-5.8(o)0(-)]TJ
-15.891 -1.1116 TD
.015 Tc
[(resce)-4.5(n)2.4(ce)-344.4(of)-343.2(targe)-4.5(t)-343.3(cells)-343(only,)-345.5(a)1.2(nd)]TJ
/F8 1 Tf
14.3993 0 TD
0 Tc
(M)Tj
/F3 1 Tf
1.1923 0 TD
.0128 Tc
[(is)-345.2(m)-6.8(a)-1(x)-5.6(i).1(mu)-5.6(m)-340.9()-5.4(uo)-5.6(re)-6.7(s-)]TJ
-15.5915 -1.1116 TD
.0188 Tc
[(ce)5(nc)5(e)-709.2(m)5(ea)5(su)6.2(re)5(d)-708(a)-.7(f)6.2(t)6.1(er)-708(l)6.1(y).4(s)6.4(i).3(n)6.2(g)-708(al)6.1(l)-708.2(c)-.7(e)5(l).3(l)6.1(s)-707.8(wi)6.1(th)-708(a)-709.2(b)6.2(uf)6.2(fe)5(r)]TJ
0 -1.1174 TD
.0131 Tc
[(c)-6.4(o).5(n)-5.3(t).4(a)-6.4(i).4(ni)-5.4(ng)-310.5(0.5)-5.3(%)-305.8(T)-6.5(r).5(i)-5.4(t).4(on)-310.5(X-)-5.2(10)-5.3(0,)-307.1(1)-5.3(0)-166.5(mM)-311.4(Tr)-5.2(is)-5.1(-H)-6.1(Cl)-310.7(\(p)-5.3(H)-305.6(7)-5.3(.4\))-5.2(,)]TJ
0 -1.1116 TD
.2951 Tc
[(a)275.6(n)282.5(d1)276.7(0)115.5(m)281.3(Mo)276.7(fE)281.3(D)275.9(T)281.3(A)281.6(.)]TJ
/F9 1 Tf
8.9663 0 0 8.9663 313.0582 526.507 Tm
0 Tc
[(Complement-dependent)-341.6(cytotoxicity)]TJ
/F3 1 Tf
9.843 0 0 9.843 323.0361 510.066 Tm
[(Cells)-290(were)-295.6(plated)-291.4(in)-284.5(96-well)-292.2(plates)-291.2(\(2)]TJ
/F10 1 Tf
15.3899 0 TD
()Tj
/F3 1 Tf
.72 0 TD
(10)Tj
7.3823 0 0 6.5614 491.4141 514.4313 Tm
(4)Tj
9.843 0 0 9.843 497.9904 510.066 Tm
[(cells/well\),)-291(in)]TJ
-18.7882 -1.1116 TD
[(DMEM)-497.9(supplemented)-501.8(with)-495.1(10%)-493.9(FBS,)-491.3(and)-494(incubated)-497.5(for)]TJ
0 -1.1174 TD
[(5)-277.6(hours)-276(at)-274.2(37)]TJ
/F10 1 Tf
5.2759 0 TD
()Tj
/F3 1 Tf
.3917 0 TD
[(C)-278.6(with)-270.5(100)]TJ
/F11 1 Tf
4.6654 0 TD
(l)Tj
/F3 1 Tf
.5529 0 TD
[(g/mL)-271.9(of)-278.6(anti-HER2)-273.6(antibodies)-278.5(or)]TJ
-10.8858 -1.1116 TD
[(control)-314.4(IgG)-309.4(\(mouse)-308.3(IgG)]TJ
7.3823 0 0 6.5614 409.7196 475.54 Tm
(2b)Tj
9.843 0 0 9.843 417.1463 477.1841 Tm
[(\))-306.3(\(Sigma-Aldrich)-313.7(Corp.\))-309.4(and)-309.7(10%)]TJ
-10.5748 -1.1116 TD
[(of)-537.8(rabbit)-532.1(complement)-535.6(\(Low-Tox-M)-539.2(Rabbit)-533.2(Complement;)]TJ
0 -1.1174 TD
[(Cedar)-8.8(lane)-185.4(Labor)-7.8(atories,)-192.1(Hornby,)-189.4(Ontario,)-192(Canada\).)-188.4(To)-193.5(assess)]TJ
0 -1.1116 TD
[(cell)-559.9(viability,)-556.6(an)-556.3(MTS)-552.4([3-\(4,5-dimethylthiazol-2-yl\)-5-\(3-)]TJ
T*
[(c)-8(a)0(r)-9.1(b)0(o)-8(x)0(y)-8(m)0(e)-10.4(t)0(h)-8.1(o)0(x)-8(y)0(p)-8(h)0(e)-9.1(n)0(y)-8(l)0(\))-8.1(-)0(2)-7.9(-)0(\()-7.9(4)0(-)-7.9(s)-6.7(ul)-8.1(fo)-7.9(ph)-8(en)-9.1(yl)-8.1(\)-)-7.9(2H)-8.8(-t)-8.1(et)-9.3(ra)-9.1(zo)-9.1(li)-8.2(um)-9.2(;)]TJ
0 -1.1174 TD
[(i)-7(n)0(n)-8(e)0(r)-331.6(s)0(a)-8.9(l)0(t)-8.2(])-323.6(a)-8(s)0(s)-7.6(a)-8(y)-323.6(w)0(a)-10(s)-323.4(p)-6.9(e)0(r)-9.1(f)0(o)-7.9(r)0(m)-9.2(e)0(d)-331.7(u)0(s)-7.8(i)0(n)-8.1(g)-323.6(a)-330.6(Cel)-11.4(lT)-9.3(it)-8.2(er)-331.6(96)-330.5(AQ)-9.7(u-)]TJ
0 -1.1116 TD
[(e)-8(o)0(u)-8(s)-283.1(a)-8(s)0(s)-7.6(a)0(y)-285.6(k)-6.9(it)-290.4(\(P)-7.8(ro)-7.9(meg)-11.5(a,)-287.9(M)-7.8(adi)-10.4(s)-6.7(o)0(n)-8(,)-279.9(W)-9.1(I)0(,)-286.7(U)0(S)-8.7(A)0(\))-8.8(.)]TJ
/F9 1 Tf
8.9663 0 0 8.9663 313.0582 388.1196 Tm
[(Antitumor)-341.4(activity)-332.8(of)-330.4(H)]TJ
6.7246 0 0 5.9768 401.6125 386.6455 Tm
(2)Tj
8.9663 0 0 8.9663 405.3542 388.1196 Tm
[(Mab-19)-331.2(in)-336.5(the)-328.5(xenografts)]TJ
-10.2937 -1.2203 TD
[(of)-336.7(colon)-332.2(cancers)]TJ
/F3 1 Tf
9.843 0 0 9.843 323.0361 360.7369 Tm
.0132 Tc
[(S)-5(i).5(x)-5.2(t).5(ee)-6.3(n)-517.8(6)-5.2(-w)-6(eek)-5.2(-o)-5.2(ld)-523.5(fe)-6.3(mal)-5.3(e)-519(B)-6.2(ALB)-6.2(/).5(c)-524.7(nud)-5.2(e)-519(m)-6.4(ice)-524.7(w)-.3(e)-6.3(r).6(e)]TJ
-1.0137 -1.1174 TD
.0166 Tc
[(pu)4(rchased)-278.2(f)-1.7(r)4(o)-1.8(m)-279.5(C)3(harl)3.9(es)-283.8(Riv)4(e)-2.9(r)-278.2(a)2.8(nd)-278.2(us)4.2(ed)-278.2(at)-284.1(7)-278.2(w)-2.6(eeks)-278.1(o)-1.8(f)-278.2(a)-2.9(g)4(e)-2.9(.)]TJ
0 -1.1116 TD
.0186 Tc
[(Ca)4.8(co)6(-2)-449(ce)4.8(ll)5.9(s)-454.6(\().3(0)6(.)3.7(3)-161(m)-1(L)-450.2(o).2(f)-454.8(1)6(.33)]TJ
/F10 1 Tf
12.8153 0 TD
0 Tc
()Tj
/F3 1 Tf
.7315 0 TD
.0184 Tc
(10)Tj
7.3823 0 0 6.5614 456.5479 343.1621 Tm
0 Tc
(8)Tj
9.843 0 0 9.843 464.9384 338.7968 Tm
.0142 Tc
[(cells/m)-5.4(L)-454.6(i)-4.3(n)-459.2(DME)-5.4(M\))]TJ
-15.4303 -1.1116 TD
.0162 Tc
[(were)-573.6(mix)3.6(e)-3.3(d)-572.4(w)2.7(it)3.5(h)-572.4(0)-2.2(.5)-163.4(mL)-579.3(BD)-573.3(Matr)3.6(i)3.5(g)-2.2(el)-578.3(Matr)3.6(ix)-572.4(Gro)3.6(w)-3(t)3.5(h)]TJ
0 -1.1174 TD
.0132 Tc
[(F)-5(a)-.6(c)-6.3(t).5(o)-5.2(r)-448.6(Re)-6.3(duc)-6.3(ed)-448.7(\()-5.1(B)-.4(D)-449.5(B)-6.2(io)-5.2(sci)-5.3(e)-.6(n)-5.2(ces)-5(,)-451(S).7(a)-6.3(n)-448.7(Jo)-5.2(se)-6.3(,)-445.2(C)-6.2(A,)-451(US)-5(A\))-5.1(.)]TJ
0 -1.1116 TD
.013 Tc
[(M)-6.3(i).3(c)-6.5(e)-588.3(w)-6.2(e)-.8(re)-594(in)-5.4(je)-6.5(ct)-5.5(ed)-587.1(s)-5.2(u).4(b)-5.4(c)-.8(u)-5.4(t).3(an)-5.4(eo)-5.4(us)-5.2(ly)-592.9(in)-5.4(to)-592.9(the)-594(l).3(e)-6.5(f).4(t)-593().6(a)-6.5(n).4(k)]TJ
T*
.0184 Tc
[(\(1)5.8(00)]TJ
/F11 1 Tf
2.062 0 TD
0 Tc
(l)Tj
/F3 1 Tf
.5702 0 TD
.0131 Tc
[(L\))-673.4(wi)-5.4(th)-673.4(th)-5.3(e)-674.6(a)-.7(fo)-5.3(re)-6.4(men)-5.3(ti)]TJ
10.6094 0 TD
.0128 Tc
[(on)-5.6(ed)-673.7(su)-5.6(sp)-5.6(ens)-5.4(i)0(o)-5.6(n)-673.7(\(5)]TJ
/F10 1 Tf
8.9103 0 TD
0 Tc
()Tj
/F3 1 Tf
.7372 0 TD
.0126 Tc
(10)Tj
7.3823 0 0 6.5614 548.5038 299.3385 Tm
0 Tc
(6)Tj
9.843 0 0 9.843 313.0582 284.0314 Tm
.0129 Tc
[(c)-6.6(e)-.9(l)-5.6(l).2(s)-5.3(\)).3(.)-226.7(A)-6.3(ft)-5.6(er)-230.1(da)-6.6(y)-224.3(1)-5.5(,)-226.7(1)-5.5(00)]TJ
/F11 1 Tf
9.6129 0 TD
0 Tc
(l)Tj
/F3 1 Tf
.5702 0 TD
.243 Tc
(gH)Tj
7.3823 0 0 6.5614 427.8046 282.3873 Tm
0 Tc
(2)Tj
9.843 0 0 9.843 431.6597 284.0314 Tm
.0175 Tc
[(Mab-)4.9(19)-225.5(o)4.9(r)-225.5(con)4.9(t)4.8(ro)4.9(l)-225.6(m)3.7(ou)4.9(se)-220.9(Ig)4.9(G)]TJ
-12.0493 -1.1174 TD
.0133 Tc
[(\()-5(S).8(i)-5.2(g).7(m)-6.3(a)-.5(-A)-5.9(ld)-5.1(ri)-5.2(ch)-379.5(C)-6.1(o).7(r)-5(p).7(.\))-379.4(i)-5.2(n)-379.5(1)-5.1(0).7(0)]TJ
/F11 1 Tf
12.8384 0 TD
0 Tc
(l)Tj
/F3 1 Tf
.5702 0 TD
.0128 Tc
[(L)-381.2(P).3(B)-6.6(S)-379.8(w)-6.4(e)-1(re)-386.9(in)-5.6(jec)-6.7(t).1(e)-6.7(d)-380(in)-5.6(to)]TJ
-13.4086 -1.1116 TD
.0129 Tc
[(t)-5.6(r).3(e)-6.6(a)-.9(t)-5.6(e)-.9(d)-339.5(a)-6.6(nd)-339.5(c)-6.6(o).3(n)-5.5(t).2(r)-5.4(o).3(l)-339.7(m)-6.7(ice)-346.5(t).2(hr)-5.4(ou)-5.5(gh)-345.3(int)-5.6(r).3(a)-6.6(p).3(e)-6.6(r).3(it)-5.6(on)-5.5(eal)-345.4(in)-5.5(jec)-6.6(t).2(i)-5.6(o).3(n)]TJ
T*
.017 Tc
[(\(i)4.3(.p.\))4.4(,)-504.8(r)4.4(es)4.6(pect)4.3(i)4.3(v)-1.4(ely)4.4(.)-504.8(Add)4.4(i)4.3(ti)4.3(on)4.4(al)-502.6(an)4.4(ti)4.3(bo)4.4(di)4.3(es)-508(were)-503.6(t)4.3(h)-1.4(en)-502.5(in)4.4(-)]TJ
0 -1.1174 TD
.0178 Tc
[(ject)5.1(ed)-328.9(o)5.2(n)-328.9(days)-328.7(7)-328.9(a)4(nd)-328.9(14)5.2(.)-331.2(E)4(ig)5.2(h)5.2(t)-.7(een)-328.9(d)5.2(ay)5.2(s)-328.7(a)-1.7(f)5.2(t)-.7(er)-328.8(cell)-329(i)5.1(m)4(pl)5.1(an)5.2(-)]TJ
0 -1.1116 TD
.0161 Tc
[(tation,)-413.5(a)2.3(ll)-411.3(mice)-412.4(w)-3.1(e)2.3(re)-412.4(euthaniz)]TJ
12.9133 0 TD
.0148 Tc
[(e)-4.7(d)-412.5(by)-418.3(cervica)-4.7(l)-412.6(d)2.2(isloca)-4.7(tion,)]TJ
-12.9133 -1.1116 TD
.0151 Tc
[(a)-4.4(n)2.5(d)-538.9(t)-3.4(umor)-533.1(dia)-4.4(meters)-538.7(a)1.3(nd)-538.9(volumes)-538.7(w)-4.1(e)1.3(re)-534.3(determ)-4.5(ined)-538.9(as)]TJ
0 -1.1174 TD
.0175 Tc
[(pr)4.9(ev)4.9(io)4.9(u)4.9(s)-.7(l)4.8(y)-277.3(descri)4.8(bed)4.9(.)]TJ
7.3823 0 0 6.5614 400.7054 211.6913 Tm
.0217 Tc
(\(10\))Tj
/F9 1 Tf
8.9663 0 0 8.9663 313.0582 183.9684 Tm
0 Tc
[(Statistical)-342.4(analyses)]TJ
/F3 1 Tf
9.843 0 0 9.843 323.0361 167.5275 Tm
-.019 Tc
[(A)-19(l).8(l)-458(d)-19(at)-19(a)-443(w)-19(er)-19(e)-448.5(e)-19(xp)-19(re)-19(ss)-19(e)-4.9(d)-452.1(a)-19(s)-448.4(me)-19(a)-6.2(n)]TJ
/F10 1 Tf
14.0018 0 TD
0 Tc
()Tj
/F3 1 Tf
.7084 0 TD
[(st)20.9(an)19.7(da)19.7(rd)-428.4(er)19.7(ro)20.9(r)-438.8(o)0(f)-422.6(t)0(h)20.7(e)]TJ
-15.724 -1.1116 TD
[(me)18.4(an)13.9(.)-228.1(S)0(t)20.9(a)0(t)19.5(i)0(s)20.9(t)0(i)14.8(c)0(a)18.5(l)-225.9(an)19.7(al)13.8(ys)21(is)-221(us)21(ed)-222.2(AN)19.1(OV)19.1(A)-226.6(a)0(n)13.9(d)-225.7(Tu)19.6(ke)19.7(y)15.1(Kr)20(am)18.5(er)19.7(s)]TJ
T*
[(te)13.8(st)-318.9(wi)19.8(th)-324.9(R)-330.4(s)0(t)20.9(a)0(t)19.6(i)0(s)20.9(t)0(i)14.8(c)0(a)18.5(l)]TJ
/F8 1 Tf
8.8123 0 TD
(p)Tj
/F10 1 Tf
.6566 0 TD
(<)Tj
/F3 1 Tf
.7084 0 TD
[(0.)18.5(05)-319(wa)18.8(s)-335(a)0(d)19.7(o)0(p)20.8(t)0(e)19.5(d)-329.4(as)-325.7(a)-330.6(l)0(e)19.5(v)0(e)19.7(l)-329.5(of)-324.7(st)20.9(a-)]TJ
-10.1774 -1.1174 TD
[(ti)14.8(st)20.9(ic)19.5(al)-262.7(si)20.9(gn)20.8(i)15.1(ca)18.5(nc)19.7(e.)]TJ
/F6 1 Tf
8.9663 0 0 8.9663 313.0582 111.3448 Tm
(Results)Tj
/F9 1 Tf
0 -1.8336 TD
[(Characterization)-341.5(of)-330.4(H)]TJ
6.7246 0 0 5.9768 397.9841 93.4299 Tm
(2)Tj
8.9663 0 0 8.9663 401.6692 94.9039 Tm
(Mab-19)Tj
-9.8827 -1.2203 TD
[(against)-340.2(colon)-332.2(cancer)-330.5(cell)-333(lines)]TJ
/F3 1 Tf
9.843 0 0 9.843 323.0361 67.5212 Tm
(H)Tj
7.3823 0 0 6.5614 330.1227 65.8771 Tm
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