%PDF-1.3
%
5 0 obj
<>>>/BBox[0 0 612 792]/Length 53>>stream
xs
w3T0WI2P0T5T R
Ɩf
!\!Y\ !p
o
endstream
endobj
1 0 obj
<>>>/BBox[0 0 612 792]/Length 53>>stream
xs
w3T0WI2P0T5T R
Ɩf
!\!Y\ !p
o
endstream
endobj
3 0 obj
<>>>/BBox[0 0 612 792]/Length 53>>stream
xs
w3T0WI2P0T5T R
Ɩf
!\!Y\ !p
o
endstream
endobj
7 0 obj
<>>>/BBox[0 0 612 792]/Length 53>>stream
xs
w3T0WI2P0T5T R
Ɩf
!\!Y\ !p
o
endstream
endobj
6 0 obj
<>>>/BBox[0 0 612 792]/Length 53>>stream
xs
w3T0WI2P0T5T R
Ɩf
!\!Y\ !p
o
endstream
endobj
4 0 obj
<>>>/BBox[0 0 612 792]/Length 53>>stream
xs
w3T0WI2P0T5T R
Ɩf
!\!Y\ !p
o
endstream
endobj
9 0 obj
<>stream
2018-10-24T06:43:49+05:30
Arbortext Advanced Print Publisher 9.0.114/W
2018-10-27T00:43:08-07:00
Acrobat Distiller 8.1.0 (Windows); modified using iText 4.2.0 by 1T3XT
application/pdf
MAB-2018-0035-ver9-Kato_2P 218..223
uuid:95cccbc4-5661-4d02-8478-0f53bba92448
uuid:e38c9596-6a32-4e73-ad99-26f99830e1c4
endstream
endobj
10 0 obj
<>stream
x+ |
endstream
endobj
11 0 obj
<>stream
xS**T0T0 Bi ye(
endstream
endobj
12 0 obj
<>stream
x+ |
endstream
endobj
13 0 obj
<>stream
xS**T0T0 Bi yJ%
endstream
endobj
14 0 obj
<>stream
x+ |
endstream
endobj
15 0 obj
<>stream
xS**T0T0 Bi yA$
endstream
endobj
16 0 obj
<>stream
x+ |
endstream
endobj
17 0 obj
<>stream
xS**T0T0 Bi yS&
endstream
endobj
18 0 obj
<>stream
x+ |
endstream
endobj
19 0 obj
<>stream
xS**T0T0 Bi y8#
endstream
endobj
20 0 obj
<>stream
x+ |
endstream
endobj
21 0 obj
<>stream
xS**T0T0 Bi y\'
endstream
endobj
23 0 obj
<>stream
/GS1 gs
BT
/F3 1 Tf
9.843 0 0 9.843 59.7543 725.1588 Tm
0 0 0 rg
-.0164 Tc
0 Tw
[(mou)5.5(s)0(e-cani)5.4(ne)-306.6(chimeri)5.4(c)-312.4(antib)5.5(odi)5.4(es)-311(f)5.6(rom)-306.7(PMab-3)5.5(8)-311.2(a)-1.4(n)5.5(d)-311.2(in)5.5(vest)5.4(i-)]TJ
0 -1.1174 TD
[(gat)5.3(e)-1.5(d)-397.7(t)-.4(h)5.4(e)-1.5(ir)-397.7(anti)5.3(body)5.4(-depen)5.4(d)-.3(en)5.4(t)-397.9(cel)5.3(lular)-397.7(c)-1.5(y)5.4(t)-.4(oto)5.4(x)-.3(icity)-397.7(\()5.5(ADCC\))]TJ
0 -1.1116 TD
[(and)-259.5(c)4.3(omplemen)5.4(t-depen)5.4(d)-.3(en)5.4(t)-259.6(c)-1.5(yt)]TJ
12.1587 0 TD
[(oto)5.5(x)-.2(icity)-259.4(\()5.6(C)-1.2(DC\))-259.4(act)5.4(ivit)5.4(ies)5.7(.)]TJ
/F7 1 Tf
8.9663 0 0 8.9663 59.7543 674.7021 Tm
0 Tc
[(Materials)-339(and)-331.9(Methods)]TJ
/F9 1 Tf
0 -1.8336 TD
[(Cell)-336.3(lines)]TJ
/F3 1 Tf
9.843 0 0 9.843 69.7323 641.8203 Tm
[(Chinese)-333.6(hamster)-328(ovary)-329.2(\(CHO\)-K1)-329.2(cell)-329.5(line)-328.4(was)-327.7(obtained)]TJ
-1.0137 -1.1116 TD
[(from)-293.5(the)-292.6(American)-291(Type)-294.8(Culture)-292.4(Collection)-291.4(\(ATCC,)-295.3(Mana-)]TJ
T*
[(ssas,)-296.4(VA\).)-290.7(In)-284.4(our)-291.3(previous)-292.1(studies,)-294.4(we)-286.5(inserted)-293.4(dPDPN)-294.9(with)]TJ
T*
[(an)-372(N-terminal)-367.1(PA)-371.5(tag)-367.5(and)-367.3(a)-365.1(C-terminal)-373(RAP)-367.9(tag-MAP)-367.7(tag)]TJ
0 -1.1174 TD
[(\(PA-dPDPN-RAP-MAP)-12.6(\))-283.3(i)0(n)-290.3(a)-284.5(pCAG-Ble)-296.1(vector)-285.5(\(FUJIFILM)]TJ
0 -1.1116 TD
[(Wako)-451.3(Pure)-448.9(Chemical)-452.6(Corporation,)-449.9(Osaka,)-449.7(Japan\).)]TJ
7.3823 0 0 6.5614 266.7968 591.4203 Tm
(\(10\))Tj
9.843 0 0 9.843 283.5212 587.0549 Tm
(The)Tj
-22.7336 -1.1116 TD
[(PA)-244.8(tag,)]TJ
7.3823 0 0 6.5614 89.2346 580.4786 Tm
(\(16\))Tj
9.843 0 0 9.843 103.9181 576.1132 Tm
[(RAP)-241.2(tag,)]TJ
7.3823 0 0 6.5614 139.9181 580.4786 Tm
(\(17\))Tj
9.843 0 0 9.843 154.6015 576.1132 Tm
[(and)-240.6(MAP)-246.8(tag)]TJ
7.3823 0 0 6.5614 206.9291 580.4786 Tm
(\(18\))Tj
9.843 0 0 9.843 221.6125 576.1132 Tm
[(consist)-244.9(of)-244.1(12)-238.3(amino)]TJ
-16.444 -1.1174 TD
[(acids)-572.2(each,)-575.8(namely,)-572.4(GVAMPGAEDDVV,)-579.4(DMVNPGLE-)]TJ
0 -1.1116 TD
[(DRIE,)-293.1(and)-286.7(GDGMVPPGIEDK,)-295.2(respectively.)-292.2(CHO-K1)-292.5(cells)]TJ
25.5097 17.3713 TD
[(were)-710.3(transfected)-719.5(with)-714(pCAG-Ble/PA-dPDPN-RAP-MAP)]TJ
0 -1.1174 TD
[(using)-454.7(Gene)-462.6(Pulser)-456.8(Xcell)-458.2(electroporation)-465.1(system)-458(\(Bio-Rad)]TJ
0 -1.1116 TD
[(Laboratories,)-390(Inc.,)-391.4(Berkeley,)-391.3(CA\))-391.1(resulting)-391.4(in)-388.2(the)-384.7(cell)-392.9(line)]TJ
T*
[(CHO/dPDPN.)-346(CHO-K1)-350.1(and)-344.3(CHO/dPDPN)-348.3(were)-341.6(cultured)-351.2(in)]TJ
T*
[(RPMI)-409.3(1640)-407.6(medium)-413.5(\(Nacalai)-412(Tesque,)-410.8(Inc.,)-414.5(Kyoto,)-407.4(Japan\))]TJ
0 -1.1174 TD
[(supplemented)-288.7(with)-282(10%)-286.6(heat-inactivated)-295.9(fetal)-284.5(bovine)-284.4(serum)]TJ
0 -1.1116 TD
[(\(Thermo)-374.1(Fisher)-370.4(Scientic,)-379.8(Inc.,)-374.2(Waltham,)-374.3(MA\),)-371.3(100)-371.9(units/)]TJ
T*
[(mL)-275.3(of)-278.6(penicillin,)-282.4(100)]TJ
/F10 1 Tf
8.7836 0 TD
(m)Tj
/F3 1 Tf
.5472 0 TD
[(g/mL)-283.4(of)-278.6(streptomycin,)-279.6(and)-280.9(25)]TJ
/F10 1 Tf
12.2279 0 TD
(m)Tj
/F3 1 Tf
.5529 0 TD
(g/mL)Tj
-22.1115 -1.1174 TD
[(of)-370.8(amphotericin)-375.4(B)-370.7(\(Nacalai)-377.4(Tesque,)-376.2(Inc.\))-371.8(at)-377.9(37)]TJ
/F5 1 Tf
19.5484 0 TD
()Tj
/F3 1 Tf
.3974 0 TD
[(C)-370.7(i)0(n)-370.9(a)-376.6(hu-)]TJ
-19.9459 -1.1116 TD
[(midied)-280.9(atmosphere)-286.4(of)-278.6(5%)-279.7(CO)]TJ
7.3823 0 0 6.5614 435.2881 624.8691 Tm
(2)Tj
9.843 0 0 9.843 441.751 626.5132 Tm
[(and)-280.9(95%)-280.8(air.)]TJ
/F9 1 Tf
8.9663 0 0 8.9663 310.8472 603.4959 Tm
(Antibodies)Tj
/F3 1 Tf
9.843 0 0 9.843 320.8251 587.0549 Tm
[(PMab-38,)-329.8(a)-330.6(mouse)-336(anti-dPDPN)-336.4(mAb)-327.9(\(IgG)]TJ
7.3823 0 0 6.5614 492.6613 585.4109 Tm
(1)Tj
9.843 0 0 9.843 496.3463 587.0549 Tm
[(,)-331.7(kappa\),)-334.9(was)]TJ
-18.8458 -1.1116 TD
[(developed)-267(as)-262.4(previously)-265.6(described.)]TJ
7.3823 0 0 6.5614 449.3479 580.4786 Tm
(\(10\))Tj
9.843 0 0 9.843 464.2581 576.1132 Tm
[(To)-262.6(generate)-267(a)-261.5(mouse-)]TJ
-15.5858 -1.1174 TD
[(canine)-194.5(\(subclass)-196.1(A\))-193.1(chimeric)-192.3(antibody,)-198(P38A,)-192.9(the)-188.9(appropriate)]TJ
0 -1.1116 TD
(V)Tj
7.3823 0 0 6.5614 317.9337 552.529 Tm
(H)Tj
9.843 0 0 9.843 325.644 554.1731 Tm
[(and)-246.4(V)]TJ
7.3823 0 0 6.5614 349.3983 552.529 Tm
(L)Tj
9.843 0 0 9.843 356.3716 554.1731 Tm
[(cDNAs)-251(of)-244.1(mouse)-249.6(PMab-38)-245.7(and)-246.4(the)-252.3(C)]TJ
7.3823 0 0 6.5614 503.7731 552.529 Tm
(H)Tj
9.843 0 0 9.843 511.54 554.1731 Tm
[(and)-246.4(C)]TJ
7.3823 0 0 6.5614 534.7841 552.529 Tm
(L)Tj
9.843 0 0 9.843 541.7573 554.1731 Tm
(of)Tj
ET
q
1 i
124.554 538.583 m
485.121 538.583 l
485.121 83.339 l
124.554 83.339 l
W n
q
360.926 0 0 455.244 124.554 83.339 cm
/Im1 Do
Q
Q
BT
/F6 1 Tf
9.845 0 0 9.845 59.7543 66.5575 Tm
[(FIG.)-325.2(1.)]TJ
/F3 1 Tf
4.1058 0 TD
[(Production)-322.2(of)-324.5(P38A)-327.1(\(canine)-321.6(IgGA\),)-326.7(P38B)-321.5(\(canine)-327.3(IgGB\),)-326.9(and)-320.9(P38Bf)-322.5(\(canine)-327.3(IgGB;)-323.7(core-fucose)-332.1(decient\))-323.6(from)]TJ
-4.1058 -1.0135 TD
[(PMab-38)-337(\(mouse)-336.3(IgG)]TJ
7.3837 0 0 6.5625 147.2315 54.9921 Tm
(1)Tj
9.845 0 0 9.845 150.9732 56.5795 Tm
(\).)Tj
/F7 1 Tf
8.9663 0 0 8.9663 59.7543 749.7069 Tm
[(MOUSE-CANINE)-340.6(CHIMERIC)-340.3(ANTI-)]TJ
6.4556 0 0 6.4556 204.0377 749.7069 Tm
(D)Tj
8.9663 0 0 8.9663 208.6866 749.7069 Tm
(PDPN)Tj
6.4556 0 0 6.4556 236.5228 749.7069 Tm
(M)Tj
8.9663 0 0 8.9663 241.9086 749.7069 Tm
(A)Tj
6.4556 0 0 6.4556 248.3716 749.7069 Tm
(B)Tj
8.9663 0 0 8.9663 535.0676 749.7069 Tm
(219)Tj
ET
endstream
endobj
26 0 obj
<>stream
Adobe d C
s !1AQa"q2B#R3b$r%C4Scs5D'6Tdt&
EFVU(eufv7GWgw8HXhx)9IYiy*:JZjz ? Nlٳf͛6lٳf͛ǯ^ЯIl4Z{E.sEs 9o0o1vJI=ǝyd5Ͳe1aU"ϓ0s(faTGpW!nn^8,bS@{D2yM}jlɧ4,]/Z(On/ZFwN`yǃ5S̺}v;
:ޞĦ`
ُ`7ȿ; ,|~еQ`Z;YRH@O%UAo|y䋈|ǫ;TW^;$jTbp>tsPP:GVߡw6lٳf͛6lٳf͛6lٳf͛6lٳf͛6lٳf͛9O&+|_hZJ`X:!~t]yoOQgFn*Ě`6~|UWX}Y=F*zCDĬ%VnĖڝDH"5E4 BQkzz=;V9aG&VIV|sM4(zQROd }ծ/]M%FwI1r0BOڡaٳf͛6lٳf͛6lٳf͛6lٳf͛6A <Pٳ sο Zt*B[o,OVGhaz~_z֟ 8y{o%$r
Dh՝C~4L 8{i~O/?Iv[''ȏG̷ 3zԧ/⭝̟yR4韗W2KW)2_'